differentiation induction  (PromoCell)

Journal: Journal of Translational Medicine

Article Title: Metabolic interplay between endometrial cancer and tumor-associated macrophages: lactate-induced M2 polarization enhances tumor progression

doi: 10.1186/s12967-025-06235-6

Figure Lengend Snippet: M2 Macrophages Enhance EC Cell Migration and Invasion. A M2 macrophages derived from lactate induction or tumor cells co-culture didn’t affect the proliferation of EC cell lines, HEC-1B and Ishikawa, by CCK8 assay. B , C Cell scratch assay demonstrated significant promotion of EC cell migration by M2 macrophages after 40 h (P < 0.05). D , E Transwell assay revealed increased EC cell invasion across Matrigel with M2 macrophage-conditioned media stimulation after 48 h (P < 0.05). F Comparison of mRNA level of EMT markers, Vimentin, CDH2, CDH1, Snail and Slug, among control, lactate-M0, and TAM three groups by RT-PCR analysis. G , H IF representative images of Vimentin, N-cadherin, and E-cadherin in the control, lactate-M0, and TAM three groups ( C ) and the expression of the above EMT markers was higher in EC cells following M2 co-culture. EC: endometrial cancer; EMT: epithelial mesenchymal transition. *P < 0.05; **P < 0.01; ***P < 0.001; ns: no significance

Article Snippet: The THP1 cell line was transfected with lentivirus to achieve IL6 knockdown, following the manufacturer's protocol, and stable knockdown cells were selected using puromycin.For M0 macrophage differentiation induction, THP-1 cells at a density of 1*10 6 cells/ml were seeded in 6-well plates stimulated with 100 ng/ml PMA (MCE, #16561-29-8) for 48 h, and then washed with PBS three times after discarding the original culture medium.

Techniques: Migration, Derivative Assay, Co-Culture Assay, CCK-8 Assay, Wound Healing Assay, Transwell Assay, Comparison, Control, Reverse Transcription Polymerase Chain Reaction, Expressing

Journal: Journal of Translational Medicine

Article Title: Metabolic interplay between endometrial cancer and tumor-associated macrophages: lactate-induced M2 polarization enhances tumor progression

doi: 10.1186/s12967-025-06235-6

Figure Lengend Snippet: EC Increased Angiogenesis via M2 Macrophages-Mediated Cytokines Release. A Comparative analysis of GSVA scores for angiogenesis in two groups of ECs, distinguished by their levels of M2 TAM infiltration. B , C Representative microscopic images (Magnification: 100 ×) showcase tube formation in control, lac-M2, Isk-M2, and HEC-M groups (Magnification: 100 ×) ( B ) and M2 macrophages significantly stimulate the formation of more capillary-like tubes (P < 0.05) ( C ). D Cytokine profile in M0, Lac-M2, and TAM-M2 conditions. E Validation of mRNA expression level of the above six cytokines of three groups. F The CCK8 assay demonstrates that GSK2837808A, an LDHA inhibitor, significantly impedes the proliferation of ECs. GSVA: gene set variation analysis; EC: endometrial cancer; TAMs: tumor-associated macrophages. *P < 0.05

Article Snippet: The THP1 cell line was transfected with lentivirus to achieve IL6 knockdown, following the manufacturer's protocol, and stable knockdown cells were selected using puromycin.For M0 macrophage differentiation induction, THP-1 cells at a density of 1*10 6 cells/ml were seeded in 6-well plates stimulated with 100 ng/ml PMA (MCE, #16561-29-8) for 48 h, and then washed with PBS three times after discarding the original culture medium.

Techniques: Control, Biomarker Discovery, Expressing, CCK-8 Assay

Journal: Frontiers in Cell and Developmental Biology

Article Title: Optimal Sca-1-based procedure for purifying mouse adipose-derived mesenchymal stem cells with enhanced proliferative and differentiation potential

doi: 10.3389/fcell.2025.1566670

Figure Lengend Snippet: Differentiation potential of mouse ADSCs obtained by three purification methods. (A) After osteogenic induction culture, the cells were stained with alizarin red S, and the histogram showed the area of calcium nodules for quantitative analysis ( n = 5). (B) Oil red O staining after adipogenic induction cultures and histogram showing lipid droplet regions for quantitative analysis ( n = 5). (C) After chondrogenic induction culture, alcian blue staining and histograms showing quantitative sorting of mucopolysaccharide regions ( n = 5). Data were analyzed by one-way ANOVA and expressed as mean ± SEM. ns, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001. Scale bar = 100 µm.

Article Snippet: In accordance with the manual for the osteogenic induction differentiation kit (Cyagen, Guangzhou, China), ADSC-A, ADSC-M, and ADSC-AM at P3 were seeded in six-well plates coated with 0.1% gelatin at a density of 2 × 10 4 cells/cm 2 .

Techniques: Purification, Staining

Journal: Frontiers in Cell and Developmental Biology

Article Title: Optimal Sca-1-based procedure for purifying mouse adipose-derived mesenchymal stem cells with enhanced proliferative and differentiation potential

doi: 10.3389/fcell.2025.1566670

Figure Lengend Snippet: Differentiation potential of mouse ADSCs obtained by three purification methods. (A) After osteogenic induction culture, the cells were stained with alizarin red S, and the histogram showed the area of calcium nodules for quantitative analysis ( n = 5). (B) Oil red O staining after adipogenic induction cultures and histogram showing lipid droplet regions for quantitative analysis ( n = 5). (C) After chondrogenic induction culture, alcian blue staining and histograms showing quantitative sorting of mucopolysaccharide regions ( n = 5). Data were analyzed by one-way ANOVA and expressed as mean ± SEM. ns, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001. Scale bar = 100 µm.

Article Snippet: In accordance with the manual for the adipogenic induction differentiation kit (Cyagen, Guangzhou, China), ADSC-A, ADSC-M, and ADSC-AM at P3 were seeded in six-well plates coated with 0.1% gelatin at a density of 2 × 10 4 cells/cm 2 .

Techniques: Purification, Staining

Journal: Frontiers in Cell and Developmental Biology

Article Title: Optimal Sca-1-based procedure for purifying mouse adipose-derived mesenchymal stem cells with enhanced proliferative and differentiation potential

doi: 10.3389/fcell.2025.1566670

Figure Lengend Snippet: Differentiation potential of mouse ADSCs obtained by three purification methods. (A) After osteogenic induction culture, the cells were stained with alizarin red S, and the histogram showed the area of calcium nodules for quantitative analysis ( n = 5). (B) Oil red O staining after adipogenic induction cultures and histogram showing lipid droplet regions for quantitative analysis ( n = 5). (C) After chondrogenic induction culture, alcian blue staining and histograms showing quantitative sorting of mucopolysaccharide regions ( n = 5). Data were analyzed by one-way ANOVA and expressed as mean ± SEM. ns, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001. Scale bar = 100 µm.

Article Snippet: In accordance with the manual for the chondrogenic induction differentiation kit (Cyagen, Guangzhou, China), ADSC-A, ADSC-M, and ADSC-AM at P3 were transferred to 15 mL tubes and centrifuged at 250 g for 4 min.

Techniques: Purification, Staining